| VEGF145

VEGF145 is an isomer of VEGF-A gene produced by variable splicing. It lacks exon 6 coding residue and retains exon 7 sequence.The human VEGF-A gene is organized in eight exons separated by seven introns and is located on chromosome 6p21.1. The coding region spans approximately 14 kb. Alternative exon splicing results in the generation of four isoforms.The four isoforms are referred to as VEGF121, VEGF165, VEGF189, and VEGF206. Less frequent splice variants, including VEGF145, VEGF183, VEGF121b, VEGF145b,VEGF165b, and VEGF189b.VEGF145 is secreted.VEGF145 has a key role during the vascularization of the human ovarian follicle and corpus luteum, in the placentation and embryonic periods, and in bone and wound healing
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.67	2.05	1.48	0.97	0.67	0.42	0.27	0.09
Corrected OD	2.58	1.96	1.39	0.88	0.58	0.33	0.18	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human VEGF145 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human VEGF145 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-96	88-96	90-110
	Average (%)	91	93	95
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	95	98	108